WOFAPS 2025 8th World Congress of Pediatric Surgery

Yin ShanFeng

Children's Hospital Zhejiang University School of Medicine, Hangzhou, China

Objective:
It is planned to further study the specific mechanism of action of KIF4A in the pathogenesis of Wilms cell carcinoma, in order to provide effective therapeutic targets for the development of new therapeutic drugs in the future.
Method:
TCGA Data Analysis:
Firstly, the genetic data of Wilms carcinoma patients in the tumor database TCGA were analyzed by using the online analysis tool UALCAN, and the significance of KIF4A for Wilms carcinoma patients was explored.
Cell Experiments:
The mature commercial cell lines 786-O, A498, OSRC-2, CaKi-1 and normal human kidney proximal convoluted tubular cells HK-2 were selected for cell experiments, and the expression of KIF4A in Wilms cell carcinoma cells compared with normal cells was explored at the level of protein and mRNA. According to the results of WB and qPCR experiments, lentiviral vectors were used to transfect cells to construct a stable cell line of Wilms cell carcinoma with KIF4A knockout or overexpression, and WB and qPCR experiments were used to verify whether KIF4A was successfully knocked out or overexpressed.
Outcome:
The data of Wilms carcinoma patients in the TCGA database were analyzed using the online analysis tools GEPIA and UALCAN, and it was found that the expression level of KIF4A in Wilms carcinoma patients was significantly increased. With the increase of cancer grade and stage, the expression level of KIF4A increased significantly. With lymph node metastasis of cancer cells, the expression level of KIF4A increases significantly.
Conclusion: The results of TCGA database analysis showed that the expression of KIF4A was positively correlated with the occurrence and development of Wilms carcinoma, suggesting that KIF4A may be a pro-tumor gene of ccRCC, and the results of cell function experiments preliminarily proved that KIF4A did promote the occurrence and development of Wilms carcinoma cells.