WOFAPS 2025 8th World Congress of Pediatric Surgery

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Poster - 182

Effects of unilateral partial ureteral obstruction on inflammatory and fibrogenetic proceses in rat kidney

Gizem Özdemir Kenar 1, Esma Kırımlıoğlu 2, Güngör Karagüzel 1
1 Akdeniz University Hospital, Faculty of Medicine, Department of Pediatric Surgery, Antalya, Turkey
2 Akdeniz University School of Medicine, Department of Histology and Embryology, Antalya, TURKEY

Aim: Although experimental models are widely used to investigate renal injury associated with ureteropelvic junction obstruction, the precise roles of inflammatory and fibrogenic pathways have not yet been elucidated. In a rat model of partial unilateral ureteral obstruction (UUO), this study aimed to explore the roles of both interleukin-6 (IL-6)-related inflammation and signal transducer and activator of transcription 3 (STAT3)-related fibrogenesis.

Materials and Methods: Eighteen Wistar rats aged 21 days were randomly divided into three groups, each group consisting of 6 rats as control (C), sham (S) and partial ureteral obstruction (PUO) groups. No intervention was performed in Group C. In Group S, the left proximal ureter was surgically exposed without obstruction. In the PUO group, the ureter was embedded in the psoas muscle at two points to induce PUO. After 14 days, all animals were sacrificed and ipsilateral nephrectomy specimens were evaluated immunohistochemically for IL-6 and STAT3 expression.

Results: In the present study, which included the early 2-week period after PUO, IL-6 expression was significantly higher in group S and PUO compared to controls (p<0.0001). However, IL-6 expression was insignificantly higher in group PUO than in group S (p>0.05). STAT3 expression was also increased in group PUO compared to groups C and S, but the difference was not significant (p>0.05).

Conclusion: The increase in IL-6 expression in the PUO group suggests a role for IL-6 in the proinflammatory response following PUO. Although not significant, the increase in STAT3 expression may reflect an early-stage fibrogenic response. Further studies with longer duration and different inflammatory/fibrogenetic markers are needed to elucidate the molecular mechanisms underlying PUO-associated renal injury.

Keywords: Kidney, ureteral obstruction, inflammation, fibrosis, STAT3, IL-6

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