WOFAPS 2025 8th World Congress of Pediatric Surgery

Deniz Yeşilli, Kaan Sönmez, Cem Kaya, Ramazan Karabulut, Zafer Türkyılmaz, Alparslan Kapısız

Department of Pediatric Surgery, Gazi University School of Medicine, Ankara, Turkey

In this study, we aim to investigate the histopathological and biochemical effects of lupeol during ovary ischemia reperfusion injury in a rat model of experimental ovarian torsion. In the study, 24 Wistar Albino rats were divided into 4 groups: Control, lupeol, ischemia and treatment. In the lupeol group, 100 mg/kg lupeol was given intraperitoneally. A bilateral ovarian torsion model was created by rotating ovaries 720 degrees clockwise and maintaining torsion for 3 hours. In the Torsion group, only torsion-detorsion was performed, whereas the Treatment group received intraperitoneal lupeol (100 mg/kg) 30 minutes before detorsion. A 24-hour reperfusion period was allowed before collecting tissue and serum samples for biochemical and histopathological evaluation.

Biochemical analysis included measurements of TAS (Total Antioxidant Status), TOS (Total Oxidant Status), OSI (Oxidative Stress Index), Caspase-3, AMH (Anti-Müllerian Hormone); histopathological assessment evaluated as follicular degeneration, hemorrhage, vascular congestion, edema which was scored for each group.Statistical analysis was performed using SPSS Version 22.0. The Treatment group showed reduced follicular degeneration, hemorrhage, vascular congestion, and edema scores compared to the Ischemia group. Tissue TOS and serum OSI levels were lower in the Treatment group than in the Ischemia group. Serum TAS (mmol/L) was higher in the Treatment group (1.34 ± 0.05) than in the Ischemia group (1.17 ± 0.07). Caspase-3 levels decreased in the Treatment group compared to the Ischemia group. Tissue AMH levels significantly increased in the Treatment group versus the Ischemia group.

This study is the first in the literature to demonstrate that lupeol exerts antioxidant, anti-apoptotic and protective effects in ovarian ischemia-reperfusion injury, while also enhancing ovarian reserve via increased AMH levels. Due to its plant-derived origin, absence of toxicity in the literature, accessibility, and low cost, lupeol holds promising clinical potential as a safe adjunctive therapy for ovarian torsion.