EN
TÜRKÇE
Poster - 165
Proximity barcoding assay reveals dynamic plasma exosome protein profiles and subpopulation characteristics in patients undergoing transcatheter pulmonary valve replacement
Runzhang Liang 1, Naijimuding Abudurexiti 2, Jing Ling 2, Jiaxiong Wu 1, Zirui Peng 2, Canxin Wang 1, Haiyun Yuan 1, Shusheng Wen 1
1 Department of Cardiovascular Surgery, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou 510080, China
2 Department of Cardiovascular Surgery, Guangdong Cardiovascular Institute, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China
Objective Patients with congenital heart disease complicated by right ventricular outflow tract obstruction (RVOTO) often develop secondary pulmonary regurgitation (PR) after corrective surgery, necessitating pulmonary valve replacement (PVR). This study employed proximity barcoding assay (PBA) to dynamically monitor changes in plasma exosome protein profiles and subpopulation characteristics during the perioperative period of transcatheter pulmonary valve replacement (TPVR).
Methods This study collected 120 human plasma samples from 10 patients undergoing TPVR at three stages, preoperative (T1 group), 1 day postoperative (T2 group), and 1 week postoperative (T3 group), as well as 10 healthy individuals (N group). Single-exosome analysis was performed using PBA. We performed combinatorial analysis and functional analysis of membrane proteins on single exosomes across different groups and evaluated their predictive performance as biomarkers using Receiver Operating Characteristic (ROC) curves. Exosome subpopulations were clustered and characterized.
Results Compared to the control group, the disease group exhibited significant downregulation of the protein combination DSCAML1 + ALCAM + ITGA1 + CR1 (P<0.05; AUC=1) and upregulation of BCAM + CD36 + DSCAML1 + ITGB1 (P<0.05; AUC>0.82). Gene Ontology (GO) analysis indicated that the proteins are associated with pathways related to response to stress, membrane side, and protein binding, while Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed enrichment in the Hematopoietic cell lineage pathway. Subpopulation analysis revealed increased cluster 1 and decreased cluster 2 proportions in T1-T3 groups compared to the N group. Subpopulation markers showed CD8A dominance in cluster 1, while DSCAML1 predominated in cluster 2.
Conclusion This study pioneers PBA-based dynamic monitoring of plasma exosomes in patients with TPVR, identifying novel diagnostic protein signatures and disease-specific subpopulation redistribution patterns.