WOFAPS 2025 8th World Congress of Pediatric Surgery

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Poster - 317

Efficacy of Single-Layer Continuous Suture in Colonic Anastomoses: A Comparative Experimental Study in a Rabbit Model

Dmitry Morozov 1, Maxim Airapetyan 2, Olga Sukhodolskaya 3
1 Veltischev Research and Clinical Institute for Pediatrics and Pediatric Surgery of the Pirogov Russian National Research Medical University
2 Moscow Multidisciplinary Medical Center
3 First Moscow State Medical University named after I.M. Sechenov (Sechenov University)

Aim:
To evaluate the safety and healing properties of single-layer continuous suturing in colonic anastomoses by analyzing clinical and histomorphological outcomes in an experimental setting.

Materials and Methods:
This study was approved by the Institutional Animal Ethics Committee. Forty-eight rabbits underwent laparotomy, colonic mobilization, and resection followed by end-to-end anastomosis. Animals were randomized into two groups: Group I (*n=24*): Single-layer continuous seromuscular-submucosal suture (excluding mucosa); Group II (*n=24*): Single-layer continuous full-thickness suture (including mucosa). Each group was further subdivided based on suture material (with/without antibacterial coating).

Macroscopic and histological assessments were performed on postoperative days 3, 7, 14, and 21. Evaluated parameters included: Restoration of intestinal motility; Adhesion severity (Evans’ scoring model); Stenosis index (Irvin’s method); Histological analysis (modified Verhofstad wound healing scale).

Results:
No mortality or anastomotic complications occurred. Key findings:

  • Adhesions: Most pronounced in Group II (full-thickness suture + non-coated thread).

  • Stenosis: Significantly lower in Group I (9.8±1.6 and 15.4±1.9) vs. Group II (21.1±2.9 and 36.2±3.5; p<0.05).

  • Histology: Precision suturing (Group I) promoted complete colonic wall regeneration. Antibacterial-coated sutures reduced inflammatory response and foreign-body reaction.

Conclusion:
Single-layer continuous seromuscular-submucosal suturing minimizes postoperative stenosis and adhesions in colonic anastomoses. Antibacterial-coated sutures further mitigate inflammation, suggesting enhanced safety and efficacy for clinical translation.

Keywords: Colonic Anastomoses, Single-Layer Continuous Suture, Experimental Study

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